Engineering of Primary Human B cells with CRISPR/Cas9 Targeted Nuclease

B cells offer unique opportunities for gene therapy because of their ability to secrete large amounts of protein in the form of antibody and persist for the life of the organism as plasma cells. Here, we report optimized CRISPR/Cas9 based genome engineering of primary human B cells. Our procedure in...

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Detalles Bibliográficos
Autores principales: Johnson, Matthew J., Laoharawee, Kanut, Lahr, Walker S., Webber, Beau R., Moriarity, Branden S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6092381/
https://www.ncbi.nlm.nih.gov/pubmed/30108345
http://dx.doi.org/10.1038/s41598-018-30358-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6092381/
https://www.ncbi.nlm.nih.gov/pubmed/30108345
http://dx.doi.org/10.1038/s41598-018-30358-0

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