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Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons

The use of genetically encoded ‘self-labeling tags’ with chemical fluorophore ligands enables rapid labeling of specific cells in neural tissue. To improve the chemical tagging of neurons, we synthesized and evaluated new fluorophore ligands based on Cy, Janelia Fluor, Alexa Fluor, and ATTO dyes and...

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Detalles Bibliográficos
Autores principales: Meissner, Geoffrey W., Grimm, Jonathan B., Johnston, Rebecca M., Sutcliffe, Ben, Ng, Julian, Jefferis, Gregory S. X. E., Cachero, Sebastian, Lavis, Luke D., Malkesman, Oz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6093644/
https://www.ncbi.nlm.nih.gov/pubmed/30110347
http://dx.doi.org/10.1371/journal.pone.0200759