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Optimization of fluorophores for chemical tagging and immunohistochemistry of Drosophila neurons
The use of genetically encoded ‘self-labeling tags’ with chemical fluorophore ligands enables rapid labeling of specific cells in neural tissue. To improve the chemical tagging of neurons, we synthesized and evaluated new fluorophore ligands based on Cy, Janelia Fluor, Alexa Fluor, and ATTO dyes and...
Autores principales: | Meissner, Geoffrey W., Grimm, Jonathan B., Johnston, Rebecca M., Sutcliffe, Ben, Ng, Julian, Jefferis, Gregory S. X. E., Cachero, Sebastian, Lavis, Luke D., Malkesman, Oz |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6093644/ https://www.ncbi.nlm.nih.gov/pubmed/30110347 http://dx.doi.org/10.1371/journal.pone.0200759 |
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