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BtsCI and BseGI display sequence preference in the nucleotides flanking the recognition sequence
Restriction enzymes are the bread and butter of Molecular Biology. Nonetheless, how restriction enzymes recognize and cleave their target is not always clear. When developing a method for the enzymatic production of oligonucleotides, we noticed that type II endonucleases BtsCI and BseGI, which recog...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6097692/ https://www.ncbi.nlm.nih.gov/pubmed/30118487 http://dx.doi.org/10.1371/journal.pone.0202057 |