Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo

Engineering of the Cpf1 crRNA has the potential to enhance its gene editing efficiency and non-viral delivery to cells. Here, we demonstrate that extending the length of its crRNA at the 5′ end can enhance the gene editing efficiency of Cpf1 both in cells and in vivo. Extending the 5′ end of the crR...

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Detalles Bibliográficos
Autores principales: Park, Hyo Min, Liu, Hui, Wu, Joann, Chong, Anthony, Mackley, Vanessa, Fellmann, Christof, Rao, Anirudh, Jiang, Fuguo, Chu, Hunghao, Murthy, Niren, Lee, Kunwoo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6098076/
https://www.ncbi.nlm.nih.gov/pubmed/30120228
http://dx.doi.org/10.1038/s41467-018-05641-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6098076/
https://www.ncbi.nlm.nih.gov/pubmed/30120228
http://dx.doi.org/10.1038/s41467-018-05641-3

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