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A Half-Century History of Applications of Antisense Oligonucleotides in Medicine, Agriculture and Forestry: We Should Continue the Journey

Antisense oligonucleotides (ASO), short single-stranded polymers based on DNA or RNA chemistries and synthesized in vitro, regulate gene expression by binding in a sequence-specific manner to an RNA target. The functional activity and selectivity in the action of ASOs largely depends on the combinat...

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Autores principales: Oberemok, Volodymyr V., Laikova, Kateryna V., Repetskaya, Anna I., Kenyo, Igor M., Gorlov, Mikhail V., Kasich, Igor N., Krasnodubets, Alisa M., Gal’chinsky, Nikita V., Fomochkina, Iryna I., Zaitsev, Aleksei S., Bekirova, Viktoriya V., Seidosmanova, Eleonora E., Dydik, Ksenia I., Meshcheryakova, Anna O., Nazarov, Sergey A., Smagliy, Natalya N., Chelengerova, Edie L., Kulanova, Alina A., Deri, Karim, Subbotkin, Mikhail V., Useinov, Refat Z., Shumskykh, Maksym N., Kubyshkin, Anatoly V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099785/
https://www.ncbi.nlm.nih.gov/pubmed/29844255
http://dx.doi.org/10.3390/molecules23061302
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author Oberemok, Volodymyr V.
Laikova, Kateryna V.
Repetskaya, Anna I.
Kenyo, Igor M.
Gorlov, Mikhail V.
Kasich, Igor N.
Krasnodubets, Alisa M.
Gal’chinsky, Nikita V.
Fomochkina, Iryna I.
Zaitsev, Aleksei S.
Bekirova, Viktoriya V.
Seidosmanova, Eleonora E.
Dydik, Ksenia I.
Meshcheryakova, Anna O.
Nazarov, Sergey A.
Smagliy, Natalya N.
Chelengerova, Edie L.
Kulanova, Alina A.
Deri, Karim
Subbotkin, Mikhail V.
Useinov, Refat Z.
Shumskykh, Maksym N.
Kubyshkin, Anatoly V.
author_facet Oberemok, Volodymyr V.
Laikova, Kateryna V.
Repetskaya, Anna I.
Kenyo, Igor M.
Gorlov, Mikhail V.
Kasich, Igor N.
Krasnodubets, Alisa M.
Gal’chinsky, Nikita V.
Fomochkina, Iryna I.
Zaitsev, Aleksei S.
Bekirova, Viktoriya V.
Seidosmanova, Eleonora E.
Dydik, Ksenia I.
Meshcheryakova, Anna O.
Nazarov, Sergey A.
Smagliy, Natalya N.
Chelengerova, Edie L.
Kulanova, Alina A.
Deri, Karim
Subbotkin, Mikhail V.
Useinov, Refat Z.
Shumskykh, Maksym N.
Kubyshkin, Anatoly V.
author_sort Oberemok, Volodymyr V.
collection PubMed
description Antisense oligonucleotides (ASO), short single-stranded polymers based on DNA or RNA chemistries and synthesized in vitro, regulate gene expression by binding in a sequence-specific manner to an RNA target. The functional activity and selectivity in the action of ASOs largely depends on the combination of nitrogenous bases in a target sequence. This simple and natural property of nucleic acids provides an attractive route by which scientists can create different ASO-based techniques. Over the last 50 years, planned and realized applications in the field of antisense and nucleic acid nanotechnologies have produced astonishing results and posed new challenges for further developments, exemplifying the essence of the post-genomic era. Today the majority of ASOs are chemically modified and/or incorporated within nanoparticles to enhance their stability and cellular uptake. This review critically analyzes some successful cases using the antisense approach in medicine to address severe diseases, such as Duchenne muscular dystrophy and spinal muscular atrophy, and suggests some prospective directions for future research. We also examine in detail the elaboration of unmodified insect-specific DNA insecticides and RNA preparations in the areas of agriculture and forestry, a relatively new branch of ASO that allows circumvention of the use of non-selective chemical insecticides. When considering the variety of successful ASO modifications with an efficient signal-to-noise ratio of action, coupled with the affordability of in vitro oligonucleotide synthesis and post-synthesis procedures, we predict that the next half-century will produce a fruitful yield of tools created from effective ASO-based end products.
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spelling pubmed-60997852018-11-13 A Half-Century History of Applications of Antisense Oligonucleotides in Medicine, Agriculture and Forestry: We Should Continue the Journey Oberemok, Volodymyr V. Laikova, Kateryna V. Repetskaya, Anna I. Kenyo, Igor M. Gorlov, Mikhail V. Kasich, Igor N. Krasnodubets, Alisa M. Gal’chinsky, Nikita V. Fomochkina, Iryna I. Zaitsev, Aleksei S. Bekirova, Viktoriya V. Seidosmanova, Eleonora E. Dydik, Ksenia I. Meshcheryakova, Anna O. Nazarov, Sergey A. Smagliy, Natalya N. Chelengerova, Edie L. Kulanova, Alina A. Deri, Karim Subbotkin, Mikhail V. Useinov, Refat Z. Shumskykh, Maksym N. Kubyshkin, Anatoly V. Molecules Review Antisense oligonucleotides (ASO), short single-stranded polymers based on DNA or RNA chemistries and synthesized in vitro, regulate gene expression by binding in a sequence-specific manner to an RNA target. The functional activity and selectivity in the action of ASOs largely depends on the combination of nitrogenous bases in a target sequence. This simple and natural property of nucleic acids provides an attractive route by which scientists can create different ASO-based techniques. Over the last 50 years, planned and realized applications in the field of antisense and nucleic acid nanotechnologies have produced astonishing results and posed new challenges for further developments, exemplifying the essence of the post-genomic era. Today the majority of ASOs are chemically modified and/or incorporated within nanoparticles to enhance their stability and cellular uptake. This review critically analyzes some successful cases using the antisense approach in medicine to address severe diseases, such as Duchenne muscular dystrophy and spinal muscular atrophy, and suggests some prospective directions for future research. We also examine in detail the elaboration of unmodified insect-specific DNA insecticides and RNA preparations in the areas of agriculture and forestry, a relatively new branch of ASO that allows circumvention of the use of non-selective chemical insecticides. When considering the variety of successful ASO modifications with an efficient signal-to-noise ratio of action, coupled with the affordability of in vitro oligonucleotide synthesis and post-synthesis procedures, we predict that the next half-century will produce a fruitful yield of tools created from effective ASO-based end products. MDPI 2018-05-29 /pmc/articles/PMC6099785/ /pubmed/29844255 http://dx.doi.org/10.3390/molecules23061302 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Oberemok, Volodymyr V.
Laikova, Kateryna V.
Repetskaya, Anna I.
Kenyo, Igor M.
Gorlov, Mikhail V.
Kasich, Igor N.
Krasnodubets, Alisa M.
Gal’chinsky, Nikita V.
Fomochkina, Iryna I.
Zaitsev, Aleksei S.
Bekirova, Viktoriya V.
Seidosmanova, Eleonora E.
Dydik, Ksenia I.
Meshcheryakova, Anna O.
Nazarov, Sergey A.
Smagliy, Natalya N.
Chelengerova, Edie L.
Kulanova, Alina A.
Deri, Karim
Subbotkin, Mikhail V.
Useinov, Refat Z.
Shumskykh, Maksym N.
Kubyshkin, Anatoly V.
A Half-Century History of Applications of Antisense Oligonucleotides in Medicine, Agriculture and Forestry: We Should Continue the Journey
title A Half-Century History of Applications of Antisense Oligonucleotides in Medicine, Agriculture and Forestry: We Should Continue the Journey
title_full A Half-Century History of Applications of Antisense Oligonucleotides in Medicine, Agriculture and Forestry: We Should Continue the Journey
title_fullStr A Half-Century History of Applications of Antisense Oligonucleotides in Medicine, Agriculture and Forestry: We Should Continue the Journey
title_full_unstemmed A Half-Century History of Applications of Antisense Oligonucleotides in Medicine, Agriculture and Forestry: We Should Continue the Journey
title_short A Half-Century History of Applications of Antisense Oligonucleotides in Medicine, Agriculture and Forestry: We Should Continue the Journey
title_sort half-century history of applications of antisense oligonucleotides in medicine, agriculture and forestry: we should continue the journey
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099785/
https://www.ncbi.nlm.nih.gov/pubmed/29844255
http://dx.doi.org/10.3390/molecules23061302
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