Simultaneous engineering of an enzyme's entrance tunnel and active site: the case of monoamine oxidase MAO-N

A new directed evolution approach is presented to enhance the activity of an enzyme and to manipulate stereoselectivity by focusing iterative saturation mutagenesis (ISM) simultaneously on residues lining the entrance tunnel and the binding pocket. This combined mutagenesis strategy was applied succ...

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Detalles Bibliográficos
Autores principales: Li, Guangyue, Yao, Peiyuan, Gong, Rui, Li, Jinlong, Liu, Pi, Lonsdale, Richard, Wu, Qiaqing, Lin, Jianping, Zhu, Dunming, Reetz, Manfred T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2017
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099926/
https://www.ncbi.nlm.nih.gov/pubmed/30155214
http://dx.doi.org/10.1039/c6sc05381e

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6099926/
https://www.ncbi.nlm.nih.gov/pubmed/30155214
http://dx.doi.org/10.1039/c6sc05381e

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