An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry

The analysis of low-level (1-100 ppm) protein impurities (e.g., host-cell proteins (HCPs)) in protein biotherapeutics is a challenging assay requiring high sensitivity and a wide dynamic range. Mass spectrometry-based quantification assays for proteins typically involve protein digestion followed by...

Descripción completa

Detalles Bibliográficos
Autores principales: Doneanu, Catalin, Fang, Jing, Alelyunas, Yun, Yu, Ying Qing, Wrona, Mark, Chen, Weibin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100639/
https://www.ncbi.nlm.nih.gov/pubmed/29733313
http://dx.doi.org/10.3791/55325

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100639/
https://www.ncbi.nlm.nih.gov/pubmed/29733313
http://dx.doi.org/10.3791/55325

Ejemplares similares