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Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

To complement existing affinity purification (AP) approaches for the identification of protein-protein interactions (PPI), enzymes have been introduced that allow the proximity-dependent labeling of proteins in living cells. One such enzyme, BirA* (used in the BioID approach), mediates the biotinyla...

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Detalles Bibliográficos
Autores principales: Schopp, Isabel M., Béthune, Julien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100705/
https://www.ncbi.nlm.nih.gov/pubmed/29733317
http://dx.doi.org/10.3791/57479