Cargando…
Insertion element mediated mgrB disruption and presence of ISKpn28 in colistin-resistant Klebsiella pneumoniae isolates from Saudi Arabia
BACKGROUND: In Klebsiella pneumoniae, mgrB and components of pmrHFIJKLM operon play a major role in colistin resistance. METHODS: We analyzed 23 nonduplicating colistin-resistant K. pneumoniae isolates, collected during the years 2011–2015, for the possible mechanism underlying their nonsusceptibili...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6101004/ https://www.ncbi.nlm.nih.gov/pubmed/30147346 http://dx.doi.org/10.2147/IDR.S161146 |
_version_ | 1783348973956759552 |
---|---|
author | Uz Zaman, Taher Albladi, Maha Siddique, Mohammed Ismail Aljohani, Sameera M Balkhy, Hanan H |
author_facet | Uz Zaman, Taher Albladi, Maha Siddique, Mohammed Ismail Aljohani, Sameera M Balkhy, Hanan H |
author_sort | Uz Zaman, Taher |
collection | PubMed |
description | BACKGROUND: In Klebsiella pneumoniae, mgrB and components of pmrHFIJKLM operon play a major role in colistin resistance. METHODS: We analyzed 23 nonduplicating colistin-resistant K. pneumoniae isolates, collected during the years 2011–2015, for the possible mechanism underlying their nonsusceptibility to colistin. Isolates were tested for their minimum inhibitory concentrations and antibiotic resistance determinants and genotyped by multilocus sequence typing (MLST). The MLST genes, antibiotic-resistant genes, and the genes of two component system (TCS), including mgrB, PhoQ/PhoP, pmrA/B, and CrrAB, were investigated by PCR amplification and Sanger sequencing. RESULTS: All isolates were distributed in eight sequence types (STs) and showed mutations either in mgrB or PhoP genes. ISKpn14 was found in 10, ISKpn28 in four, and IS903 in three isolates. One isolate showed deletion of a single nucleotide in mgrB open reading frame causing premature stop codon. L26Q substitution in PhoP was found in five isolates. CONCLUSION: The mutations in mgrB were mostly mediated by insertion elements (IS). ISKpn14 is the major IS while ISKpn28 is reported for the first time in mediating mgrB disruption. IS903, an IS5 family member, involved in mgrB disruption in three ST-152 NDM-1-positive isolates, was previously responsible for omp-36 disruption in our carbapenem-resistant K. pneumoniae and appears to contribute to transform the isolates into a pan-drug ones. Also, the abundance of insertion sites in mgrB indicates the plasticity of this gene. In our isolates, IS-mediated colistin resistance appears to be a later phenomenon than mutation in PhoP gene. |
format | Online Article Text |
id | pubmed-6101004 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-61010042018-08-24 Insertion element mediated mgrB disruption and presence of ISKpn28 in colistin-resistant Klebsiella pneumoniae isolates from Saudi Arabia Uz Zaman, Taher Albladi, Maha Siddique, Mohammed Ismail Aljohani, Sameera M Balkhy, Hanan H Infect Drug Resist Original Research BACKGROUND: In Klebsiella pneumoniae, mgrB and components of pmrHFIJKLM operon play a major role in colistin resistance. METHODS: We analyzed 23 nonduplicating colistin-resistant K. pneumoniae isolates, collected during the years 2011–2015, for the possible mechanism underlying their nonsusceptibility to colistin. Isolates were tested for their minimum inhibitory concentrations and antibiotic resistance determinants and genotyped by multilocus sequence typing (MLST). The MLST genes, antibiotic-resistant genes, and the genes of two component system (TCS), including mgrB, PhoQ/PhoP, pmrA/B, and CrrAB, were investigated by PCR amplification and Sanger sequencing. RESULTS: All isolates were distributed in eight sequence types (STs) and showed mutations either in mgrB or PhoP genes. ISKpn14 was found in 10, ISKpn28 in four, and IS903 in three isolates. One isolate showed deletion of a single nucleotide in mgrB open reading frame causing premature stop codon. L26Q substitution in PhoP was found in five isolates. CONCLUSION: The mutations in mgrB were mostly mediated by insertion elements (IS). ISKpn14 is the major IS while ISKpn28 is reported for the first time in mediating mgrB disruption. IS903, an IS5 family member, involved in mgrB disruption in three ST-152 NDM-1-positive isolates, was previously responsible for omp-36 disruption in our carbapenem-resistant K. pneumoniae and appears to contribute to transform the isolates into a pan-drug ones. Also, the abundance of insertion sites in mgrB indicates the plasticity of this gene. In our isolates, IS-mediated colistin resistance appears to be a later phenomenon than mutation in PhoP gene. Dove Medical Press 2018-08-15 /pmc/articles/PMC6101004/ /pubmed/30147346 http://dx.doi.org/10.2147/IDR.S161146 Text en © 2018 Uz Zaman et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Uz Zaman, Taher Albladi, Maha Siddique, Mohammed Ismail Aljohani, Sameera M Balkhy, Hanan H Insertion element mediated mgrB disruption and presence of ISKpn28 in colistin-resistant Klebsiella pneumoniae isolates from Saudi Arabia |
title | Insertion element mediated mgrB disruption and presence of ISKpn28 in colistin-resistant Klebsiella pneumoniae isolates from Saudi Arabia |
title_full | Insertion element mediated mgrB disruption and presence of ISKpn28 in colistin-resistant Klebsiella pneumoniae isolates from Saudi Arabia |
title_fullStr | Insertion element mediated mgrB disruption and presence of ISKpn28 in colistin-resistant Klebsiella pneumoniae isolates from Saudi Arabia |
title_full_unstemmed | Insertion element mediated mgrB disruption and presence of ISKpn28 in colistin-resistant Klebsiella pneumoniae isolates from Saudi Arabia |
title_short | Insertion element mediated mgrB disruption and presence of ISKpn28 in colistin-resistant Klebsiella pneumoniae isolates from Saudi Arabia |
title_sort | insertion element mediated mgrb disruption and presence of iskpn28 in colistin-resistant klebsiella pneumoniae isolates from saudi arabia |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6101004/ https://www.ncbi.nlm.nih.gov/pubmed/30147346 http://dx.doi.org/10.2147/IDR.S161146 |
work_keys_str_mv | AT uzzamantaher insertionelementmediatedmgrbdisruptionandpresenceofiskpn28incolistinresistantklebsiellapneumoniaeisolatesfromsaudiarabia AT albladimaha insertionelementmediatedmgrbdisruptionandpresenceofiskpn28incolistinresistantklebsiellapneumoniaeisolatesfromsaudiarabia AT siddiquemohammedismail insertionelementmediatedmgrbdisruptionandpresenceofiskpn28incolistinresistantklebsiellapneumoniaeisolatesfromsaudiarabia AT aljohanisameeram insertionelementmediatedmgrbdisruptionandpresenceofiskpn28incolistinresistantklebsiellapneumoniaeisolatesfromsaudiarabia AT balkhyhananh insertionelementmediatedmgrbdisruptionandpresenceofiskpn28incolistinresistantklebsiellapneumoniaeisolatesfromsaudiarabia |