Investigating mycobacterial topoisomerase I mechanism from the analysis of metal and DNA substrate interactions at the active site

We have obtained new crystal structures of Mycobacterium tuberculosis topoisomerase I, including structures with ssDNA substrate bound to the active site, with and without Mg(2+) ion present. Significant enzyme conformational changes upon DNA binding place the catalytic tyrosine in a pre-transition...

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Detalles Bibliográficos
Autores principales: Cao, Nan, Tan, Kemin, Annamalai, Thirunavukkarasu, Joachimiak, Andrzej, Tse-Dinh, Yuk-Ching
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6101483/
https://www.ncbi.nlm.nih.gov/pubmed/29905859
http://dx.doi.org/10.1093/nar/gky492

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6101483/
https://www.ncbi.nlm.nih.gov/pubmed/29905859
http://dx.doi.org/10.1093/nar/gky492

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