Targeting protein-protein interactions for therapeutic discovery via FRET-based high-throughput screening in living cells

We have developed a structure-based high-throughput screening (HTS) method, using time-resolved fluorescence resonance energy transfer (TR-FRET) that is sensitive to protein-protein interactions in living cells. The membrane protein complex between the cardiac sarcoplasmic reticulum Ca-ATPase (SERCA...

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Detalles Bibliográficos
Autores principales: Stroik, Daniel R., Yuen, Samantha L., Janicek, Kevyn A., Schaaf, Tory M., Li, Ji, Ceholski, Delaine K., Hajjar, Roger J., Cornea, Razvan L., Thomas, David D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6105598/
https://www.ncbi.nlm.nih.gov/pubmed/30135432
http://dx.doi.org/10.1038/s41598-018-29685-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6105598/
https://www.ncbi.nlm.nih.gov/pubmed/30135432
http://dx.doi.org/10.1038/s41598-018-29685-z

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