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Memory B cells are reactivated in subcapsular proliferative foci of lymph nodes

Vaccine-induced immunity depends on the generation of memory B cells (MBC). However, where and how MBCs are reactivated to make neutralising antibodies remain unknown. Here we show that MBCs are prepositioned in a subcapsular niche in lymph nodes where, upon reactivation by antigen, they rapidly pro...

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Autores principales: Moran, Imogen, Nguyen, Akira, Khoo, Weng Hua, Butt, Danyal, Bourne, Katherine, Young, Clara, Hermes, Jana R., Biro, Maté, Gracie, Gary, Ma, Cindy S., Munier, C. Mee Ling, Luciani, Fabio, Zaunders, John, Parker, Andrew, Kelleher, Anthony D., Tangye, Stuart G., Croucher, Peter I., Brink, Robert, Read, Mark N., Phan, Tri Giang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6105623/
https://www.ncbi.nlm.nih.gov/pubmed/30135429
http://dx.doi.org/10.1038/s41467-018-05772-7
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author Moran, Imogen
Nguyen, Akira
Khoo, Weng Hua
Butt, Danyal
Bourne, Katherine
Young, Clara
Hermes, Jana R.
Biro, Maté
Gracie, Gary
Ma, Cindy S.
Munier, C. Mee Ling
Luciani, Fabio
Zaunders, John
Parker, Andrew
Kelleher, Anthony D.
Tangye, Stuart G.
Croucher, Peter I.
Brink, Robert
Read, Mark N.
Phan, Tri Giang
author_facet Moran, Imogen
Nguyen, Akira
Khoo, Weng Hua
Butt, Danyal
Bourne, Katherine
Young, Clara
Hermes, Jana R.
Biro, Maté
Gracie, Gary
Ma, Cindy S.
Munier, C. Mee Ling
Luciani, Fabio
Zaunders, John
Parker, Andrew
Kelleher, Anthony D.
Tangye, Stuart G.
Croucher, Peter I.
Brink, Robert
Read, Mark N.
Phan, Tri Giang
author_sort Moran, Imogen
collection PubMed
description Vaccine-induced immunity depends on the generation of memory B cells (MBC). However, where and how MBCs are reactivated to make neutralising antibodies remain unknown. Here we show that MBCs are prepositioned in a subcapsular niche in lymph nodes where, upon reactivation by antigen, they rapidly proliferate and differentiate into antibody-secreting plasma cells in the subcapsular proliferative foci (SPF). This novel structure is enriched for signals provided by T follicular helper cells and antigen-presenting subcapsular sinus macrophages. Compared with contemporaneous secondary germinal centres, SPF have distinct single-cell molecular signature, cell migration pattern and plasma cell output. Moreover, SPF are found both in human and mouse lymph nodes, suggesting that they are conserved throughout mammalian evolution. Our data thus reveal that SPF is a seat of immunological memory that may be exploited to rapidly mobilise secondary antibody responses and improve vaccine efficacy.
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spelling pubmed-61056232018-08-27 Memory B cells are reactivated in subcapsular proliferative foci of lymph nodes Moran, Imogen Nguyen, Akira Khoo, Weng Hua Butt, Danyal Bourne, Katherine Young, Clara Hermes, Jana R. Biro, Maté Gracie, Gary Ma, Cindy S. Munier, C. Mee Ling Luciani, Fabio Zaunders, John Parker, Andrew Kelleher, Anthony D. Tangye, Stuart G. Croucher, Peter I. Brink, Robert Read, Mark N. Phan, Tri Giang Nat Commun Article Vaccine-induced immunity depends on the generation of memory B cells (MBC). However, where and how MBCs are reactivated to make neutralising antibodies remain unknown. Here we show that MBCs are prepositioned in a subcapsular niche in lymph nodes where, upon reactivation by antigen, they rapidly proliferate and differentiate into antibody-secreting plasma cells in the subcapsular proliferative foci (SPF). This novel structure is enriched for signals provided by T follicular helper cells and antigen-presenting subcapsular sinus macrophages. Compared with contemporaneous secondary germinal centres, SPF have distinct single-cell molecular signature, cell migration pattern and plasma cell output. Moreover, SPF are found both in human and mouse lymph nodes, suggesting that they are conserved throughout mammalian evolution. Our data thus reveal that SPF is a seat of immunological memory that may be exploited to rapidly mobilise secondary antibody responses and improve vaccine efficacy. Nature Publishing Group UK 2018-08-22 /pmc/articles/PMC6105623/ /pubmed/30135429 http://dx.doi.org/10.1038/s41467-018-05772-7 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Moran, Imogen
Nguyen, Akira
Khoo, Weng Hua
Butt, Danyal
Bourne, Katherine
Young, Clara
Hermes, Jana R.
Biro, Maté
Gracie, Gary
Ma, Cindy S.
Munier, C. Mee Ling
Luciani, Fabio
Zaunders, John
Parker, Andrew
Kelleher, Anthony D.
Tangye, Stuart G.
Croucher, Peter I.
Brink, Robert
Read, Mark N.
Phan, Tri Giang
Memory B cells are reactivated in subcapsular proliferative foci of lymph nodes
title Memory B cells are reactivated in subcapsular proliferative foci of lymph nodes
title_full Memory B cells are reactivated in subcapsular proliferative foci of lymph nodes
title_fullStr Memory B cells are reactivated in subcapsular proliferative foci of lymph nodes
title_full_unstemmed Memory B cells are reactivated in subcapsular proliferative foci of lymph nodes
title_short Memory B cells are reactivated in subcapsular proliferative foci of lymph nodes
title_sort memory b cells are reactivated in subcapsular proliferative foci of lymph nodes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6105623/
https://www.ncbi.nlm.nih.gov/pubmed/30135429
http://dx.doi.org/10.1038/s41467-018-05772-7
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