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Use of coagulation factor XIII (F13) gene as an internal control for normalization of genomic DNA’s for HLA typing

Genomic DNA (gDNA) obtained from whole blood samples is a critical element for genomic research and clinical diagnosis. PCR efficiencies of the targeted genes like HLA-A, -B, -C, DPB1 and DRB1 using such isolated gDNAs were variable in spite of having similar amounts of gDNA taken for PCR. We addres...

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Detalles Bibliográficos
Autores principales: Neduvat, Anupama Cheleri, Murthy, Prerana Madhusudhana, Sundarrajan, Sudarson, Padmanabhan, Sriram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107889/
https://www.ncbi.nlm.nih.gov/pubmed/30151348
http://dx.doi.org/10.1016/j.mex.2018.07.020