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Use of coagulation factor XIII (F13) gene as an internal control for normalization of genomic DNA’s for HLA typing
Genomic DNA (gDNA) obtained from whole blood samples is a critical element for genomic research and clinical diagnosis. PCR efficiencies of the targeted genes like HLA-A, -B, -C, DPB1 and DRB1 using such isolated gDNAs were variable in spite of having similar amounts of gDNA taken for PCR. We addres...
Autores principales: | Neduvat, Anupama Cheleri, Murthy, Prerana Madhusudhana, Sundarrajan, Sudarson, Padmanabhan, Sriram |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107889/ https://www.ncbi.nlm.nih.gov/pubmed/30151348 http://dx.doi.org/10.1016/j.mex.2018.07.020 |
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