Cargando…

Measurement of copy number variation in single cancer cells using rapid-emulsification digital droplet MDA

Uniform amplification of low-input DNA is important for applications across biology, including single-cell genomics, forensic science, and microbial and viral sequencing. However, the requisite biochemical amplification methods are prone to bias, skewing sequence proportions and obscuring signals re...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kim, Samuel C., Premasekharan, Gayatri, Clark, Iain C., Gemeda, Hawi B., Paris, Pamela L., Abate, Adam R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6108428/ https://www.ncbi.nlm.nih.gov/pubmed/30147985 http://dx.doi.org/10.1038/micronano.2017.18

Ejemplares similares

Particle-Templated Emulsification for Microfluidics-Free Digital Biology
por: Hatori, Makiko N., et al.
Publicado: (2018)

Rapid screening of copy number variations in STRC by droplet digital PCR in patients with mild-to-moderate hearing loss
por: Ito, Taku, et al.
Publicado: (2019)

Quantification of Plasmid Copy Number with Single Colour Droplet Digital PCR
por: Plotka, Magdalena, et al.
Publicado: (2017)

Emulsification of Surfactant on Oil Droplets by Molecular Dynamics Simulation
por: Cheng, Yaoshuang, et al.
Publicado: (2020)

Copy Number Variation and Rearrangements Assessment in Cancer: Comparison of Droplet Digital PCR with the Current Approaches
por: Cusenza, Vincenza Ylenia, et al.
Publicado: (2021)

High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number
por: Hindson, Benjamin J., et al.
Publicado: (2011)

Establishment and Validation of an Integrated Microfluidic Step Emulsification Chip Supporting Droplet Digital Nucleic Acid Analysis
por: Luo, Gangyin, et al.
Publicado: (2023)

Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR
por: Jahn, Michael, et al.
Publicado: (2016)

Killer-cell Immunoglobulin-like Receptor gene linkage and copy number variation analysis by droplet digital PCR
por: Roberts, Chrissy h, et al.
Publicado: (2014)

Recent Progress of Droplet Microfluidic Emulsification Based Synthesis of Functional Microparticles
por: Long, Fei, et al.
Publicado: (2023)

DNA copy number concentration measured by digital and droplet digital quantitative PCR using certified reference materials
por: Corbisier, Philippe, et al.
Publicado: (2015)

Evaluation of a Droplet Digital Polymerase Chain Reaction Format for DNA Copy Number Quantification
por: Pinheiro, Leonardo B., et al.
Publicado: (2011)

Detection of MET Gene Copy Number in Cancer Samples Using the Droplet Digital PCR Method
por: Zhang, Yanni, et al.
Publicado: (2016)

High-throughput single-cell whole-genome amplification through centrifugal emulsification and eMDA
por: Fu, Yusi, et al.
Publicado: (2019)

Microfluidic EDGE emulsification: the importance of interface interactions on droplet formation and pressure stability
por: Sahin, Sami, et al.
Publicado: (2016)

Versatile Tool for Droplet Generation in Standard Reaction Tubes by Centrifugal Step Emulsification
por: Schulz, Martin, et al.
Publicado: (2020)

Gas-assisted microfluidic step-emulsification for generating micron- and submicron-sized droplets
por: Huang, Biao, et al.
Publicado: (2023)

Application of Droplet Digital PCR for Estimating Vector Copy Number States in Stem Cell Gene Therapy
por: Lin, Huan-Ting, et al.
Publicado: (2016)

Using droplet digital PCR to analyze MYCN and ALK copy number in plasma from patients with neuroblastoma
por: Lodrini, Marco, et al.
Publicado: (2017)

Quantitative mitochondrial DNA copy number determination using droplet digital PCR with single-cell resolution
por: O'Hara, Ryan, et al.
Publicado: (2019)

Application of droplet digital PCR for the detection of vector copy number in clinical CAR/TCR T cell products
por: Lu, Alex, et al.
Publicado: (2020)

Array Comparative Genomic Hybridisation and Droplet Digital PCR Uncover Recurrent Copy Number Variation of the TTN Segmental Duplication Region
por: Sagath, Lydia, et al.
Publicado: (2022)

Microfluidics-free single-cell genomics with templated emulsification
por: Clark, Iain C., et al.
Publicado: (2023)

Numerical simulations of wall contact angle effects on droplet size during step emulsification
por: Wang, Meng, et al.
Publicado: (2018)

Experimental and numerical analysis of the emulsification of oil droplets in water with high frequency focused ultrasound
por: Adeyemi, Idowu, et al.
Publicado: (2023)

Droplet digital PCR shows the D-Loop to be an error prone locus for mitochondrial DNA copy number determination
por: Li, Brian, et al.
Publicado: (2018)

Detection of clinically relevant copy number alterations in oral cancer progression using multiplexed droplet digital PCR
por: Hughesman, Curtis B., et al.
Publicado: (2017)

Clinically Applicable Assessment of Tisagenlecleucel CAR T Cell Treatment by Digital Droplet PCR for Copy Number Variant Assessment
por: Gkazi, Soragia Athina, et al.
Publicado: (2022)

Detection and quantification of integrated vector copy number by multiplex droplet digital PCR in dual-transduced CAR T cells
por: Wang, Wei, et al.
Publicado: (2023)

Relationship between clinical features and droplet digital PCR copy number in non-HIV patients with pneumocystis pneumonia
por: Bian, Wenjie, et al.
Publicado: (2023)

High Sensitivity Detection and Quantitation of DNA Copy Number and Single Nucleotide Variants with Single Color Droplet Digital PCR
por: Miotke, Laura, et al.
Publicado: (2014)

Microfluidic Coupling of Step Emulsification and Deterministic Lateral Displacement for Producing Satellite-Free Droplets and Particles
por: Ji, Guangchong, et al.
Publicado: (2023)

Digital PCR for the Analysis of MYC Copy Number Variation in Lung Cancer
por: Brik, Alexander, et al.
Publicado: (2020)

Correction to High Sensitivity Detection and Quantitation of DNA Copy Number and Single Nucleotide Variants with Single Color Droplet Digital PCR
por: Miotke, Laura, et al.
Publicado: (2014)

Correction to High Sensitivity Detection and Quantitation of DNA Copy Number and Single Nucleotide Variants with Single Color Droplet Digital PCR
por: Miotke, Laura, et al.
Publicado: (2015)

A Robust Protocol for Using Multiplexed Droplet Digital PCR to Quantify Somatic Copy Number Alterations in Clinical Tissue Specimens
por: Hughesman, Curtis B., et al.
Publicado: (2016)

Comparative analysis of HER2 copy number between plasma and tissue samples in gastric cancer using droplet digital PCR
por: Kim, Boram, et al.
Publicado: (2020)

Development and Evaluation of a Droplet Digital PCR Assay for 8p23 β-Defensin Cluster Copy Number Determination
por: Wen, Tingting, et al.
Publicado: (2021)

Investigation of Factors Influencing Formation of Nanoemulsion by Spontaneous Emulsification: Impact on Droplet Size, Polydispersity Index, and Stability
por: Algahtani, Mohammed S., et al.
Publicado: (2022)

Copy number determination of the gene for the human pancreatic polypeptide receptor NPY4R using read depth analysis and droplet digital PCR
por: Shebanits, Kateryna, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_3t8e420ihm0ecgbepkgkcfi428