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Biological serial block face scanning electron microscopy at improved z-resolution based on Monte Carlo model
Serial block-face electron microscopy (SBEM) provides nanoscale 3D ultrastructure of embedded and stained cells and tissues in volumes of up to 10(7) µm(3). In SBEM, electrons with 1–3 keV energies are incident on a specimen block, from which backscattered electron (BSE) images are collected with x,...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6113311/ https://www.ncbi.nlm.nih.gov/pubmed/30154532 http://dx.doi.org/10.1038/s41598-018-31231-w |