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Thapsigargin Increases IL-2 Production in T Cells at Nanomolar Concentrations
Thapsigargin (TGN) is a potent and selective inhibitor of sarco-endoplasmic Ca(2+)-ATPase, leading to rapid elevation of cytoplasmic Ca(2+) concentration. Previous reports have shown that TGN increases the production of various cytokines from macrophages and dendritic cells. Here, we examine the eff...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Association of Immunologists
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117510/ https://www.ncbi.nlm.nih.gov/pubmed/30181914 http://dx.doi.org/10.4110/in.2018.18.e26 |
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author | Kim, Ki-Hyang Kim, Sang-Hyun Jung, Ho-Hyun Moon, Jun-Hyeok Jeong, Seong-Un Yu, Kyeongae Lee, Chong-Kil |
author_facet | Kim, Ki-Hyang Kim, Sang-Hyun Jung, Ho-Hyun Moon, Jun-Hyeok Jeong, Seong-Un Yu, Kyeongae Lee, Chong-Kil |
author_sort | Kim, Ki-Hyang |
collection | PubMed |
description | Thapsigargin (TGN) is a potent and selective inhibitor of sarco-endoplasmic Ca(2+)-ATPase, leading to rapid elevation of cytoplasmic Ca(2+) concentration. Previous reports have shown that TGN increases the production of various cytokines from macrophages and dendritic cells. Here, we examine the effects of TGN on murine T cells. Nanomolar concentrations of TGN are a significant inducer of IL-2 production with full activity at 50 nM. Micromolar concentrations of TGN, however, are inhibitory to IL-2 production and T cell proliferation. The IL-2 production-inducing activity of TGN is much more prominent when T cells are primed with concanavalin A or anti-CD3 mAb, and is due to the increase of cytoplasmic Ca(2+) concentration. TGN at 50 nM does not affect interferon-gamma or IL-4 production from T cells. Thus, the present study shows that low nanomolar concentrations of TGN could be useful in potentiating IL-2 production from antigen-primed T cells. |
format | Online Article Text |
id | pubmed-6117510 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Korean Association of Immunologists |
record_format | MEDLINE/PubMed |
spelling | pubmed-61175102018-09-04 Thapsigargin Increases IL-2 Production in T Cells at Nanomolar Concentrations Kim, Ki-Hyang Kim, Sang-Hyun Jung, Ho-Hyun Moon, Jun-Hyeok Jeong, Seong-Un Yu, Kyeongae Lee, Chong-Kil Immune Netw Brief Communication Thapsigargin (TGN) is a potent and selective inhibitor of sarco-endoplasmic Ca(2+)-ATPase, leading to rapid elevation of cytoplasmic Ca(2+) concentration. Previous reports have shown that TGN increases the production of various cytokines from macrophages and dendritic cells. Here, we examine the effects of TGN on murine T cells. Nanomolar concentrations of TGN are a significant inducer of IL-2 production with full activity at 50 nM. Micromolar concentrations of TGN, however, are inhibitory to IL-2 production and T cell proliferation. The IL-2 production-inducing activity of TGN is much more prominent when T cells are primed with concanavalin A or anti-CD3 mAb, and is due to the increase of cytoplasmic Ca(2+) concentration. TGN at 50 nM does not affect interferon-gamma or IL-4 production from T cells. Thus, the present study shows that low nanomolar concentrations of TGN could be useful in potentiating IL-2 production from antigen-primed T cells. The Korean Association of Immunologists 2018-08-14 /pmc/articles/PMC6117510/ /pubmed/30181914 http://dx.doi.org/10.4110/in.2018.18.e26 Text en Copyright © 2018. The Korean Association of Immunologists https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Brief Communication Kim, Ki-Hyang Kim, Sang-Hyun Jung, Ho-Hyun Moon, Jun-Hyeok Jeong, Seong-Un Yu, Kyeongae Lee, Chong-Kil Thapsigargin Increases IL-2 Production in T Cells at Nanomolar Concentrations |
title | Thapsigargin Increases IL-2 Production in T Cells at Nanomolar Concentrations |
title_full | Thapsigargin Increases IL-2 Production in T Cells at Nanomolar Concentrations |
title_fullStr | Thapsigargin Increases IL-2 Production in T Cells at Nanomolar Concentrations |
title_full_unstemmed | Thapsigargin Increases IL-2 Production in T Cells at Nanomolar Concentrations |
title_short | Thapsigargin Increases IL-2 Production in T Cells at Nanomolar Concentrations |
title_sort | thapsigargin increases il-2 production in t cells at nanomolar concentrations |
topic | Brief Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117510/ https://www.ncbi.nlm.nih.gov/pubmed/30181914 http://dx.doi.org/10.4110/in.2018.18.e26 |
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