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A Genetically Encoded Biosensor Strategy for Quantifying Non-muscle Myosin II Phosphorylation Dynamics in Living Cells and Organisms

Complex cell behaviors require dynamic control over non-muscle myosin II (NMMII) regulatory light chain (RLC) phosphorylation. Here, we report that RLC phosphorylation can be tracked in living cells and organisms using a homotransfer fluorescence resonance energy transfer (FRET) approach. Fluorescen...

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Detalles Bibliográficos
Autores principales: Markwardt, Michele L., Snell, Nicole E., Guo, Min, Wu, Yicong, Christensen, Ryan, Liu, Huafeng, Shroff, Hari, Rizzo, M.A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117825/
https://www.ncbi.nlm.nih.gov/pubmed/30044973
http://dx.doi.org/10.1016/j.celrep.2018.06.088