Cargando…
A Genetically Encoded Biosensor Strategy for Quantifying Non-muscle Myosin II Phosphorylation Dynamics in Living Cells and Organisms
Complex cell behaviors require dynamic control over non-muscle myosin II (NMMII) regulatory light chain (RLC) phosphorylation. Here, we report that RLC phosphorylation can be tracked in living cells and organisms using a homotransfer fluorescence resonance energy transfer (FRET) approach. Fluorescen...
Autores principales: | Markwardt, Michele L., Snell, Nicole E., Guo, Min, Wu, Yicong, Christensen, Ryan, Liu, Huafeng, Shroff, Hari, Rizzo, M.A. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6117825/ https://www.ncbi.nlm.nih.gov/pubmed/30044973 http://dx.doi.org/10.1016/j.celrep.2018.06.088 |
Ejemplares similares
-
Single-color, ratiometric biosensors for detecting signaling activities in live cells
por: Ross, Brian L, et al.
Publicado: (2018) -
Multiscale fluorescence imaging of living samples
por: Wu, Yicong, et al.
Publicado: (2022) -
Filamentous smooth muscle myosin is regulated by phosphorylation
Publicado: (1989) -
Phosphorylation of myosin regulatory light chain controls myosin head conformation in cardiac muscle
por: Kampourakis, Thomas, et al.
Publicado: (2015) -
Quantifying myosin light chain phosphorylation in single adherent cells with automated fluorescence microscopy
por: Bhadriraju, Kiran, et al.
Publicado: (2007)