Cargando…

Construction of an overexpression library for Mycobacterium tuberculosis

There is a pressing need to develop novel anti-tubercular drugs. High-throughput phenotypic screening yields chemical series that inhibit bacterial growth. Target identification for such series is challenging, but necessary for optimization of target engagement and the development of series into cli...

Descripción completa

Detalles Bibliográficos
Autores principales:	Melief, Eduard, Kokoczka, Rachel, Files, Megan, Bailey, Mai Ann, Alling, Torey, Li, Hongye, Ahn, James, Misquith, Ayesha, Korkegian, Aaron, Roberts, David, Sacchettini, James, Parish, Tanya
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2018
Materias:	Methods Manuscript
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6118195/ https://www.ncbi.nlm.nih.gov/pubmed/30197930 http://dx.doi.org/10.1093/biomethods/bpy009

Ejemplares similares

The 4-aminopiperidine series has limited anti-tubercular and anti-staphylococcus aureus activity
por: Chandrasekera, N Susantha, et al.
Publicado: (2015)

Identification of the translational start site of codon-optimized mCherry in Mycobacterium tuberculosis
por: Carroll, Paul, et al.
Publicado: (2014)

In Vitro Evaluation of Novel Nitazoxanide Derivatives against Mycobacterium tuberculosis
por: Odingo, Joshua, et al.
Publicado: (2017)

Synthesis and anti-tubercular activity of 3-substituted benzo[b]thiophene-1,1-dioxides
por: Chandrasekera, N. Susantha, et al.
Publicado: (2014)

A Dual Read-Out Assay to Evaluate the Potency of Compounds Active against Mycobacterium tuberculosis
por: Ollinger, Juliane, et al.
Publicado: (2013)

8‐Hydroxyquinolines are bactericidal against Mycobacterium tuberculosis
por: Odingo, Joshua O., et al.
Publicado: (2019)

Optimization and Evaluation of 5-Styryl-Oxathiazol-2-one Mycobacterium tuberculosis Proteasome Inhibitors as Potential Antitubercular Agents
por: Russo, Francesco, et al.
Publicado: (2015)

PrimerPooler: automated primer pooling to prepare library for targeted sequencing
por: Brown, Silas S., et al.
Publicado: (2017)

LytB1 and LytB2 of Mycobacterium tuberculosis Are Not Genetically Redundant
por: Brown, Amanda Claire, et al.
Publicado: (2015)

Homology-based enzymatic DNA fragment assembly-based illumina sequencing library preparation
por: Shinozuka, Hiroshi, et al.
Publicado: (2018)

Identification of cyclic hexapeptides natural products with inhibitory potency against Mycobacterium tuberculosis
por: Singh, Sheo B., et al.
Publicado: (2018)

InhA inhibitors have activity against non-replicating Mycobacterium tuberculosis
por: Flint, Lindsay, et al.
Publicado: (2020)

Imidazoles Induce Reactive Oxygen Species in Mycobacterium tuberculosis Which Is Not Associated with Cell Death
por: Howell Wescott, Heather A., et al.
Publicado: (2017)

Activation of 2,4-Diaminoquinazoline in Mycobacterium tuberculosis by Rv3161c, a Putative Dioxygenase
por: Melief, Eduard, et al.
Publicado: (2018)

A Target-Based Whole Cell Screen Approach To Identify Potential Inhibitors of Mycobacterium tuberculosis Signal Peptidase
por: Bonnett, Shilah A., et al.
Publicado: (2016)

Leveraging modern DNA assembly techniques for rapid, markerless genome modification
por: Tikh, Ilya B, et al.
Publicado: (2016)

Novel chemical entities inhibiting Mycobacterium tuberculosis growth identified by phenotypic high-throughput screening
por: Kumar, Anuradha, et al.
Publicado: (2022)

The 7-phenyl benzoxaborole series is active against Mycobacterium tuberculosis
por: Korkegian, Aaron, et al.
Publicado: (2018)

A Novel 6-Benzyl Ether Benzoxaborole Is Active against Mycobacterium tuberculosis In Vitro
por: Patel, Nipul, et al.
Publicado: (2017)

Identification of Compounds with pH-Dependent Bactericidal Activity against Mycobacterium tuberculosis
por: Early, Julie, et al.
Publicado: (2018)

Assessment of configurations and chemistries of bridged nucleic acids-containing oligomers as external guide sequences: a methodology for inhibition of expression of antibiotic resistance genes
por: Jackson, Alexis, et al.
Publicado: (2016)

A method for the efficient and selective identification of 5-hydroxymethyluracil in genomic DNA
por: Bullard, Whitney, et al.
Publicado: (2017)

Immunofluorescence and image analysis pipeline for Drosophila motor neurons
por: Brown, Jeremy R, et al.
Publicado: (2019)

Live imaging of intracellular pH in planarians using the ratiometric fluorescent dye SNARF-5F-AM
por: Beane, Wendy Scott, et al.
Publicado: (2019)

Characterization of FFPE-induced bacterial DNA damage and development of a repair method
por: Flores Bueso, Yensi, et al.
Publicado: (2020)

Fine-mapping within eQTL credible intervals by expression CROP-seq
por: Pan, Yidan, et al.
Publicado: (2020)

Optimization of Chelex 100 resin-based extraction of genomic DNA from dried blood spots
por: Simon, Neta, et al.
Publicado: (2020)

ACValidator: A novel assembly-based approach for in silico verification of circular RNAs
por: Sekar, Shobana, et al.
Publicado: (2020)

A rapid and low-cost method for genomic DNA extraction from the cyanobacterium Synechocystis
por: Harrison, Dale J A, et al.
Publicado: (2020)

Pharmacophore-driven identification of N-methyl-D-receptor antagonists as potent neuroprotective agents validated using in vivo studies
por: Sharma, Mukta, et al.
Publicado: (2020)

Method combining BAC film and positive staining for the characterization of DNA intermediates by dark-field electron microscopy
por: Benureau, Yann, et al.
Publicado: (2020)

Rapid and inexpensive whole-genome sequencing of SARS-CoV-2 using 1200 bp tiled amplicons and Oxford Nanopore Rapid Barcoding
por: Freed, Nikki E, et al.
Publicado: (2020)

An array-based melt curve analysis method for the identification and classification of closely related pathogen strains
por: Hassibi, Arjang, et al.
Publicado: (2018)

Assessment of six commercial plasma small RNA isolation kits using qRT-PCR and electrophoretic separation: higher recovery of microRNA following ultracentrifugation
por: Meerson, Ari, et al.
Publicado: (2016)

Improving the efficiency of gene insertion in a human artificial chromosome vector and its transfer in human-induced pluripotent stem cells
por: Hasegawa, Yoshinori, et al.
Publicado: (2018)

Identification of Penicillium species by MALDI-TOF MS analysis of spores collected by dielectrophoresis
por: Reeve, Michael A, et al.
Publicado: (2019)

A fast, sensitive and cost-effective method for nucleic acid detection using non-radioactive probes
por: Viterbo, David, et al.
Publicado: (2018)

Assessment of phagocytic activity in live macrophages-tumor cells co-cultures by Confocal and Nomarski Microscopy
por: Martinez-Marin, Dalia, et al.
Publicado: (2017)

qPCR-based characterization of DNA fragmentation efficiency of Tn5 transposomes
por: Rykalina, Vera, et al.
Publicado: (2017)

A cost-effective technique for generating preservable biomass smoke extract and measuring its effect on cell receptor expression in human bronchial epithelial cells
por: Rajendra, K C, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_a62f1fj22jiaasn0e080s5t50o