A rapid in vitro method to flip back the double-floxed inverted open reading frame in a plasmid

BACKGROUND: The LoxP site based genetic switch, the FLEX, also known as DIO (Double-Floxed Inverted Open reading frame), was invented to turn on gene expression via Cre-mediated recombination. Since its first publication, numerous FLEX switch plasmids have been generated. These plasmids are designed...

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Detalles Bibliográficos
Autores principales: Xu, Jian, Zhu, Yongling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6119287/
https://www.ncbi.nlm.nih.gov/pubmed/30170595
http://dx.doi.org/10.1186/s12896-018-0462-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6119287/
https://www.ncbi.nlm.nih.gov/pubmed/30170595
http://dx.doi.org/10.1186/s12896-018-0462-x

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