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A rapid in vitro method to flip back the double-floxed inverted open reading frame in a plasmid
BACKGROUND: The LoxP site based genetic switch, the FLEX, also known as DIO (Double-Floxed Inverted Open reading frame), was invented to turn on gene expression via Cre-mediated recombination. Since its first publication, numerous FLEX switch plasmids have been generated. These plasmids are designed...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2018
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6119287/ https://www.ncbi.nlm.nih.gov/pubmed/30170595 http://dx.doi.org/10.1186/s12896-018-0462-x |