Triple Fluorescence staining to Evaluate Mechanism-based Apoptosis following Chemotherapeutic and Targeted Anti-cancer Drugs in Live Tumor Cells

We present a protocol for live cancer cell-imaging by triple-fluorescent staining to test 3 crucial mechanisms of apoptosis; the enzymatic activity of executioner caspase3, caspase-dependent phosphatidylserine presentation on the cell surface and mitochondrial function. We standardized a protocol to...

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Detalles Bibliográficos
Autores principales: De, Pradip, Carlson, Jennifer H., Leyland-Jones, Brian, Williams, Casey, Dey, Nandini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123436/
https://www.ncbi.nlm.nih.gov/pubmed/30181562
http://dx.doi.org/10.1038/s41598-018-31575-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123436/
https://www.ncbi.nlm.nih.gov/pubmed/30181562
http://dx.doi.org/10.1038/s41598-018-31575-3

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