Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion

The ability to precisely edit individual bases of bacterial genomes would accelerate the investigation of the function of genes. Here we utilized a nickase Cas9-cytidine deaminase fusion protein to direct the conversion of cytosine to thymine within prokaryotic cells, resulting in high mutagenesis f...

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Detalles Bibliográficos
Autores principales: Zheng, Ke, Wang, Yang, Li, Na, Jiang, Fang-Fang, Wu, Chang-Xian, Liu, Fang, Chen, Huan-Chun, Liu, Zheng-Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123677/
https://www.ncbi.nlm.nih.gov/pubmed/30271918
http://dx.doi.org/10.1038/s42003-018-0035-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123677/
https://www.ncbi.nlm.nih.gov/pubmed/30271918
http://dx.doi.org/10.1038/s42003-018-0035-5

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