Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion

The ability to precisely edit individual bases of bacterial genomes would accelerate the investigation of the function of genes. Here we utilized a nickase Cas9-cytidine deaminase fusion protein to direct the conversion of cytosine to thymine within prokaryotic cells, resulting in high mutagenesis f...

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Detalles Bibliográficos
Autores principales: Zheng, Ke, Wang, Yang, Li, Na, Jiang, Fang-Fang, Wu, Chang-Xian, Liu, Fang, Chen, Huan-Chun, Liu, Zheng-Fei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123677/
https://www.ncbi.nlm.nih.gov/pubmed/30271918
http://dx.doi.org/10.1038/s42003-018-0035-5
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author Zheng, Ke
Wang, Yang
Li, Na
Jiang, Fang-Fang
Wu, Chang-Xian
Liu, Fang
Chen, Huan-Chun
Liu, Zheng-Fei
author_facet Zheng, Ke
Wang, Yang
Li, Na
Jiang, Fang-Fang
Wu, Chang-Xian
Liu, Fang
Chen, Huan-Chun
Liu, Zheng-Fei
author_sort Zheng, Ke
collection PubMed
description The ability to precisely edit individual bases of bacterial genomes would accelerate the investigation of the function of genes. Here we utilized a nickase Cas9-cytidine deaminase fusion protein to direct the conversion of cytosine to thymine within prokaryotic cells, resulting in high mutagenesis frequencies in Escherichia coli and Brucella melitensis. Our study suggests that CRISPR/Cas9-guided base-editing is a viable alternative approach to generate mutant bacterial strains.
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spelling pubmed-61236772018-09-28 Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion Zheng, Ke Wang, Yang Li, Na Jiang, Fang-Fang Wu, Chang-Xian Liu, Fang Chen, Huan-Chun Liu, Zheng-Fei Commun Biol Article The ability to precisely edit individual bases of bacterial genomes would accelerate the investigation of the function of genes. Here we utilized a nickase Cas9-cytidine deaminase fusion protein to direct the conversion of cytosine to thymine within prokaryotic cells, resulting in high mutagenesis frequencies in Escherichia coli and Brucella melitensis. Our study suggests that CRISPR/Cas9-guided base-editing is a viable alternative approach to generate mutant bacterial strains. Nature Publishing Group UK 2018-04-19 /pmc/articles/PMC6123677/ /pubmed/30271918 http://dx.doi.org/10.1038/s42003-018-0035-5 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Zheng, Ke
Wang, Yang
Li, Na
Jiang, Fang-Fang
Wu, Chang-Xian
Liu, Fang
Chen, Huan-Chun
Liu, Zheng-Fei
Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion
title Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion
title_full Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion
title_fullStr Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion
title_full_unstemmed Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion
title_short Highly efficient base editing in bacteria using a Cas9-cytidine deaminase fusion
title_sort highly efficient base editing in bacteria using a cas9-cytidine deaminase fusion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123677/
https://www.ncbi.nlm.nih.gov/pubmed/30271918
http://dx.doi.org/10.1038/s42003-018-0035-5
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