Light-activated cell identification and sorting (LACIS) for selection of edited clones on a nanofluidic device

Despite improvements in the CRISPR molecular toolbox, identifying and purifying properly edited clones remains slow, laborious, and low-yield. Here, we establish a method to enable clonal isolation, selection, and expansion of properly edited cells, using OptoElectroPositioning technology for single...

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Detalles Bibliográficos
Autores principales: Mocciaro, Annamaria, Roth, Theodore L., Bennett, Hayley M., Soumillon, Magali, Shah, Abhik, Hiatt, Joseph, Chapman, Kevin, Marson, Alexander, Lavieu, Gregory
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123811/
https://www.ncbi.nlm.nih.gov/pubmed/30271925
http://dx.doi.org/10.1038/s42003-018-0034-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123811/
https://www.ncbi.nlm.nih.gov/pubmed/30271925
http://dx.doi.org/10.1038/s42003-018-0034-6

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