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Light-activated cell identification and sorting (LACIS) for selection of edited clones on a nanofluidic device
Despite improvements in the CRISPR molecular toolbox, identifying and purifying properly edited clones remains slow, laborious, and low-yield. Here, we establish a method to enable clonal isolation, selection, and expansion of properly edited cells, using OptoElectroPositioning technology for single...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2018
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123811/ https://www.ncbi.nlm.nih.gov/pubmed/30271925 http://dx.doi.org/10.1038/s42003-018-0034-6 |