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Application of whole genome data for in silico evaluation of primers and probes routinely employed for the detection of viral species by RT-qPCR using dengue virus as a case study

BACKGROUND: Viral infection by dengue virus is a major public health problem in tropical countries. Early diagnosis and detection are increasingly based on quantitative reverse transcriptase real-time polymerase chain reaction (RT-qPCR) directed against genomic regions conserved between different is...

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Detalles Bibliográficos
Autores principales: Vanneste, Kevin, Garlant, Linda, Broeders, Sylvia, Van Gucht, Steven, Roosens, Nancy H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6123964/
https://www.ncbi.nlm.nih.gov/pubmed/30180800
http://dx.doi.org/10.1186/s12859-018-2313-0