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Efficient genome editing using CRISPR/Cas9 ribonucleoprotein approach in cultured Medaka fish cells

Gene editing with CRISPR/Cas9 is a powerful tool to study the function of target genes. Although this technology has demonstrated wide efficiency in many species, including fertilized zebrafish and medaka fish embryos when microinjected, its application to achieve efficient gene editing in cultured...

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Detalles Bibliográficos
Autores principales: Liu, Qizhi, Yuan, Yongming, Zhu, Feng, Hong, Yunhan, Ge, Ruowen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6124564/
https://www.ncbi.nlm.nih.gov/pubmed/30072445
http://dx.doi.org/10.1242/bio.035170