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tRNA tracking for direct measurements of protein synthesis kinetics in live cells

Our ability to directly relate results from test tube biochemical experiments to the kinetics in living cells is very limited. Here we present experimental and analytical tools to directly study the kinetics of fast biochemical reactions in live cells. Dye-labeled molecules are electroporated into b...

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Detalles Bibliográficos
Autores principales: Volkov, Ivan L., Lindén, Martin, Rivera, Javier Aguirre, Ieong, Ka-Weng, Metelev, Mikhail, Elf, Johan, Johansson, Magnus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6124642/
https://www.ncbi.nlm.nih.gov/pubmed/29769736
http://dx.doi.org/10.1038/s41589-018-0063-y