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A two-hybrid antibody micropattern assay reveals specific in cis interactions of MHC I heavy chains at the cell surface

We demonstrate a two-hybrid assay based on antibody micropatterns to study protein-protein interactions at the cell surface of major histocompatibility complex class I (MHC I) proteins. Anti-tag and conformation-specific antibodies are used for individual capture of specific forms of MHC I proteins...

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Detalles Bibliográficos
Autores principales: Dirscherl, Cindy, Hein, Zeynep, Ramnarayan, Venkat Raman, Jacob-Dolan, Catherine, Springer, Sebastian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6125123/
https://www.ncbi.nlm.nih.gov/pubmed/30180933
http://dx.doi.org/10.7554/eLife.34150