Efficient single-copy HDR by 5’ modified long dsDNA donors

CRISPR/Cas9 efficiently induces targeted mutations via non-homologous-end-joining but for genome editing, precise, homology-directed repair (HDR) of endogenous DNA stretches is a prerequisite. To favor HDR, many approaches interfere with the repair machinery or manipulate Cas9 itself. Using Medaka w...

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Detalles Bibliográficos
Autores principales: Gutierrez-Triana, Jose Arturo, Tavhelidse, Tinatini, Thumberger, Thomas, Thomas, Isabelle, Wittbrodt, Beate, Kellner, Tanja, Anlas, Kerim, Tsingos, Erika, Wittbrodt, Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2018
Materias:
Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6125127/
https://www.ncbi.nlm.nih.gov/pubmed/30156184
http://dx.doi.org/10.7554/eLife.39468

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6125127/
https://www.ncbi.nlm.nih.gov/pubmed/30156184
http://dx.doi.org/10.7554/eLife.39468

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