Conditional accumulation of toxic tRNAs to cause amino acid misincorporation

To develop a system for conditional amino acid misincorporation, we engineered tRNAs in the yeast Saccharomyces cerevisiae to be substrates of the rapid tRNA decay (RTD) pathway, such that they accumulate when RTD is turned off. We used this system to test the effects on growth of a library of tRNA(...

Descripción completa

Detalles Bibliográficos
Autores principales: Zimmerman, Stephanie M, Kon, Yoshiko, Hauke, Alayna C, Ruiz, Bianca Y, Fields, Stanley, Phizicky, Eric M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2018
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6125640/
https://www.ncbi.nlm.nih.gov/pubmed/30007351
http://dx.doi.org/10.1093/nar/gky623

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6125640/
https://www.ncbi.nlm.nih.gov/pubmed/30007351
http://dx.doi.org/10.1093/nar/gky623

Ejemplares similares