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Estimating the frequency of multiplets in single-cell RNA sequencing from cell-mixing experiments

In single-cell RNA-sequencing, it is important to know the frequency at which the sequenced transcriptomes actually derive from multiple cells. A common method to estimate this multiplet frequency is to mix two different types of cells (e.g., human and mouse), and then determine how often the transc...

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Detalles Bibliográficos
Autor principal: Bloom, Jesse D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126471/
https://www.ncbi.nlm.nih.gov/pubmed/30202659
http://dx.doi.org/10.7717/peerj.5578