Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

In vivo, proteins are often part of large macromolecular complexes where binding specificity and dynamics ultimately dictate functional outputs. In this work, the pre-endosomal anthrax toxin is assembled and transitioned into the endosomal complex. First, the N-terminal domain of a cysteine mutant l...

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Detalles Bibliográficos
Autores principales: Machen, Alexandra J., O'Neil, Pierce T., Pentelute, Bradley L., Villar, Maria T., Artigues, Antonio, Fisher, Mark T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2018
Materias:
Biochemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126661/
https://www.ncbi.nlm.nih.gov/pubmed/30124667
http://dx.doi.org/10.3791/57902

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126661/
https://www.ncbi.nlm.nih.gov/pubmed/30124667
http://dx.doi.org/10.3791/57902

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