Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction

Base editors enable targeted single-nucleotide conversion in genomic DNA. Here we show that expression levels are a bottleneck in base editing efficiency. We optimize cytidine (BE4) and adenine (ABE7.10) base editors by modification of nuclear localization signals and codon usage, and ancestral reco...

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Detalles Bibliográficos
Autores principales: Koblan, Luke W., Doman, Jordan L., Wilson, Christopher, Levy, Jonathan M., Tay, Tristan, Newby, Greg A., Maianti, Juan Pablo, Raguram, Aditya, Liu, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126947/
https://www.ncbi.nlm.nih.gov/pubmed/29813047
http://dx.doi.org/10.1038/nbt.4172

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6126947/
https://www.ncbi.nlm.nih.gov/pubmed/29813047
http://dx.doi.org/10.1038/nbt.4172

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