Novel properties of recombinant Sso7d-Taq DNA polymerase purified using aqueous two-phase extraction: Utilities of the enzyme in viral diagnosis

Using Sso7d from Sulfolobus solfataricus as the DNA binding protein fused to Taq DNA polymerase at its amino terminus, we report the hyper-expression and a novel purification methodology of Sso7d-Taq polymerase (S-Taq) using aqueous two-phase extraction system followed by Ni-affinity chromatography....

Descripción completa

Detalles Bibliográficos
Autores principales: Sundarrajan, Sudarson, Parambath, Sreesada, Suresh, Swetha, Rao, Sneha, Padmanabhan, Sriram
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6127375/
https://www.ncbi.nlm.nih.gov/pubmed/30197870
http://dx.doi.org/10.1016/j.btre.2018.e00270

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6127375/
https://www.ncbi.nlm.nih.gov/pubmed/30197870
http://dx.doi.org/10.1016/j.btre.2018.e00270

Ejemplares similares