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Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction
The lack of an effective, simple, and highly sensitive protocol for fluorescent in situ hybridization (FISH) at the Drosophila larval neuromuscular junction (NMJ) has hampered the study of mRNA biology. Here, we describe our modified single molecule FISH (smFISH) methods that work well in whole moun...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer New York
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6128253/ https://www.ncbi.nlm.nih.gov/pubmed/29130196 http://dx.doi.org/10.1007/978-1-4939-7213-5_10 |