Plasmid-Based One-Pot Saturation Mutagenesis and Robot-Based Automated Screening for Protein Engineering

[Image: see text] We evaluated a method for protein engineering using plasmid-based one-pot saturation mutagenesis and robot-based automated screening. When the biases in nucleotides and amino acids were assessed for a loss-of-function point mutation in green fluorescent protein, the ratios of gain-...

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Detalles Bibliográficos
Autores principales: Kawai, Fumihiro, Nakamura, Akihiko, Visootsat, Akasit, Iino, Ryota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2018
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130897/
https://www.ncbi.nlm.nih.gov/pubmed/30221239
http://dx.doi.org/10.1021/acsomega.8b00663

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6130897/
https://www.ncbi.nlm.nih.gov/pubmed/30221239
http://dx.doi.org/10.1021/acsomega.8b00663

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