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PAX5A and PAX5B isoforms are both efficient to drive B cell differentiation

Pax5 is the guardian of the B cell identity since it primes or enhances the expression of B cell specific genes and concomitantly represses the expression of B cell inappropriate genes. The tight regulation of Pax5 is therefore required for an efficient B cell differentiation. A defect in its dosage...

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Autores principales: Cresson, Charlotte, Péron, Sophie, Jamrog, Laura, Rouquié, Nelly, Prade, Nais, Dubois, Marine, Hébrard, Sylvie, Lagarde, Stéphanie, Gerby, Bastien, Mancini, Stéphane J.C., Cogné, Michel, Delabesse, Eric, Delpy, Laurent, Broccardo, Cyril
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6132355/
https://www.ncbi.nlm.nih.gov/pubmed/30214688
http://dx.doi.org/10.18632/oncotarget.26003
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author Cresson, Charlotte
Péron, Sophie
Jamrog, Laura
Rouquié, Nelly
Prade, Nais
Dubois, Marine
Hébrard, Sylvie
Lagarde, Stéphanie
Gerby, Bastien
Mancini, Stéphane J.C.
Cogné, Michel
Delabesse, Eric
Delpy, Laurent
Broccardo, Cyril
author_facet Cresson, Charlotte
Péron, Sophie
Jamrog, Laura
Rouquié, Nelly
Prade, Nais
Dubois, Marine
Hébrard, Sylvie
Lagarde, Stéphanie
Gerby, Bastien
Mancini, Stéphane J.C.
Cogné, Michel
Delabesse, Eric
Delpy, Laurent
Broccardo, Cyril
author_sort Cresson, Charlotte
collection PubMed
description Pax5 is the guardian of the B cell identity since it primes or enhances the expression of B cell specific genes and concomitantly represses the expression of B cell inappropriate genes. The tight regulation of Pax5 is therefore required for an efficient B cell differentiation. A defect in its dosage can translate into immunodeficiency or malignant disorders such as leukemia or lymphoma. Pax5 is expressed from two different promoters encoding two isoforms that only differ in the sequence of their first alternative exon. Very little is known regarding the role of the two isoforms during B cell differentiation and the regulation of their expression. Our work aims to characterize the mechanisms of regulation of the expression balance of these two isoforms and their implication in the B cell differentiation process using murine ex vivo analyses. We show that these two isoforms are differentially regulated but have equivalent function during early B cell differentiation and may have functional differences after B cell activation. The tight control of their expression may thus reflect a way to finely tune Pax5 dosage during B cell differentiation process.
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spelling pubmed-61323552018-09-13 PAX5A and PAX5B isoforms are both efficient to drive B cell differentiation Cresson, Charlotte Péron, Sophie Jamrog, Laura Rouquié, Nelly Prade, Nais Dubois, Marine Hébrard, Sylvie Lagarde, Stéphanie Gerby, Bastien Mancini, Stéphane J.C. Cogné, Michel Delabesse, Eric Delpy, Laurent Broccardo, Cyril Oncotarget Research Paper Pax5 is the guardian of the B cell identity since it primes or enhances the expression of B cell specific genes and concomitantly represses the expression of B cell inappropriate genes. The tight regulation of Pax5 is therefore required for an efficient B cell differentiation. A defect in its dosage can translate into immunodeficiency or malignant disorders such as leukemia or lymphoma. Pax5 is expressed from two different promoters encoding two isoforms that only differ in the sequence of their first alternative exon. Very little is known regarding the role of the two isoforms during B cell differentiation and the regulation of their expression. Our work aims to characterize the mechanisms of regulation of the expression balance of these two isoforms and their implication in the B cell differentiation process using murine ex vivo analyses. We show that these two isoforms are differentially regulated but have equivalent function during early B cell differentiation and may have functional differences after B cell activation. The tight control of their expression may thus reflect a way to finely tune Pax5 dosage during B cell differentiation process. Impact Journals LLC 2018-08-28 /pmc/articles/PMC6132355/ /pubmed/30214688 http://dx.doi.org/10.18632/oncotarget.26003 Text en Copyright: © 2018 Cresson et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Cresson, Charlotte
Péron, Sophie
Jamrog, Laura
Rouquié, Nelly
Prade, Nais
Dubois, Marine
Hébrard, Sylvie
Lagarde, Stéphanie
Gerby, Bastien
Mancini, Stéphane J.C.
Cogné, Michel
Delabesse, Eric
Delpy, Laurent
Broccardo, Cyril
PAX5A and PAX5B isoforms are both efficient to drive B cell differentiation
title PAX5A and PAX5B isoforms are both efficient to drive B cell differentiation
title_full PAX5A and PAX5B isoforms are both efficient to drive B cell differentiation
title_fullStr PAX5A and PAX5B isoforms are both efficient to drive B cell differentiation
title_full_unstemmed PAX5A and PAX5B isoforms are both efficient to drive B cell differentiation
title_short PAX5A and PAX5B isoforms are both efficient to drive B cell differentiation
title_sort pax5a and pax5b isoforms are both efficient to drive b cell differentiation
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6132355/
https://www.ncbi.nlm.nih.gov/pubmed/30214688
http://dx.doi.org/10.18632/oncotarget.26003
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