Filamentation and restoration of normal growth in Escherichia coli using a combined CRISPRi sgRNA/antisense RNA approach

CRISPR interference (CRISPRi) using dCas9-sgRNA is a powerful tool for the exploration and manipulation of gene functions. Here we quantify the reversible switching of a central process of the bacterial cell cycle by CRISPRi and an antisense RNA mechanism. Reversible induction of filamentous growth...

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Detalles Bibliográficos
Autores principales: Mückl, Andrea, Schwarz-Schilling, Matthaeus, Fischer, Katrin, Simmel, Friedrich C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6133276/
https://www.ncbi.nlm.nih.gov/pubmed/30204770
http://dx.doi.org/10.1371/journal.pone.0198058

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6133276/
https://www.ncbi.nlm.nih.gov/pubmed/30204770
http://dx.doi.org/10.1371/journal.pone.0198058

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