Establishment of an Efficient and Flexible Genetic Manipulation Platform Based on a Fosmid Library for Rapid Generation of Recombinant Pseudorabies Virus

Conventional genetic engineering of pseudorabies virus (PRV) is essentially based on homologous recombination or bacterial artificial chromosome. However, these techniques require multiple plaque purification, which is labor-intensive and time-consuming. The aim of the present study was to develop a...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhou, Mo, Abid, Muhammad, Yin, Hang, Wu, Hongxia, Teklue, Teshale, Qiu, Hua-Ji, Sun, Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6133995/
https://www.ncbi.nlm.nih.gov/pubmed/30233561
http://dx.doi.org/10.3389/fmicb.2018.02132

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6133995/
https://www.ncbi.nlm.nih.gov/pubmed/30233561
http://dx.doi.org/10.3389/fmicb.2018.02132

Ejemplares similares