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Protein fishing from single live cells

Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to ‘fish’ native cytoplasmic or...

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Detalles Bibliográficos
Autores principales: Shekaramiz, Elaheh, Doshi, Rupak, Wickramasinghe, H. Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134770/
https://www.ncbi.nlm.nih.gov/pubmed/30205820
http://dx.doi.org/10.1186/s12951-018-0395-5