Protein fishing from single live cells

Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to ‘fish’ native cytoplasmic or...

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Detalles Bibliográficos
Autores principales: Shekaramiz, Elaheh, Doshi, Rupak, Wickramasinghe, H. Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2018
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134770/
https://www.ncbi.nlm.nih.gov/pubmed/30205820
http://dx.doi.org/10.1186/s12951-018-0395-5
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author Shekaramiz, Elaheh
Doshi, Rupak
Wickramasinghe, H. Kumar
author_facet Shekaramiz, Elaheh
Doshi, Rupak
Wickramasinghe, H. Kumar
author_sort Shekaramiz, Elaheh
collection PubMed
description Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to ‘fish’ native cytoplasmic or nuclear proteins from single mammalian cells, without compromising cell viability, followed by ex cellulo quantitative detection. Our work paves the way for spatiotemporally-controlled, quantitative, live, single-cell proteomics. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12951-018-0395-5) contains supplementary material, which is available to authorized users.
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spelling pubmed-61347702018-09-15 Protein fishing from single live cells Shekaramiz, Elaheh Doshi, Rupak Wickramasinghe, H. Kumar J Nanobiotechnology Short Communication Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to ‘fish’ native cytoplasmic or nuclear proteins from single mammalian cells, without compromising cell viability, followed by ex cellulo quantitative detection. Our work paves the way for spatiotemporally-controlled, quantitative, live, single-cell proteomics. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12951-018-0395-5) contains supplementary material, which is available to authorized users. BioMed Central 2018-09-11 /pmc/articles/PMC6134770/ /pubmed/30205820 http://dx.doi.org/10.1186/s12951-018-0395-5 Text en © The Author(s) 2018 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Communication
Shekaramiz, Elaheh
Doshi, Rupak
Wickramasinghe, H. Kumar
Protein fishing from single live cells
title Protein fishing from single live cells
title_full Protein fishing from single live cells
title_fullStr Protein fishing from single live cells
title_full_unstemmed Protein fishing from single live cells
title_short Protein fishing from single live cells
title_sort protein fishing from single live cells
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134770/
https://www.ncbi.nlm.nih.gov/pubmed/30205820
http://dx.doi.org/10.1186/s12951-018-0395-5
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