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Protein fishing from single live cells
Intracellular protein and proteomic studies using mass spectrometry, imaging microscopy, flow cytometry, or western blotting techniques require genetic manipulation, cell permeabilization, and/or cell lysis. We present a biophysical method that employs a nanoaspirator to ‘fish’ native cytoplasmic or...
Autores principales: | Shekaramiz, Elaheh, Doshi, Rupak, Wickramasinghe, H. Kumar |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6134770/ https://www.ncbi.nlm.nih.gov/pubmed/30205820 http://dx.doi.org/10.1186/s12951-018-0395-5 |
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