Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells

Oligodendrocyte dysfunction underlies many neurological disorders, but rapid assessment of mutation-specific effects in these cells has been impractical. To enable functional genetics in oligodendrocytes, here we report a highly efficient method for generating oligodendrocytes and their progenitors...

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Autores principales: Lager, Angela M., Corradin, Olivia G., Cregg, Jared M., Elitt, Matthew S., Shick, H. Elizabeth, Clayton, Benjamin L. L., Allan, Kevin C., Olsen, Hannah E., Madhavan, Mayur, Tesar, Paul J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6137209/
https://www.ncbi.nlm.nih.gov/pubmed/30213958
http://dx.doi.org/10.1038/s41467-018-06102-7
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author Lager, Angela M.
Corradin, Olivia G.
Cregg, Jared M.
Elitt, Matthew S.
Shick, H. Elizabeth
Clayton, Benjamin L. L.
Allan, Kevin C.
Olsen, Hannah E.
Madhavan, Mayur
Tesar, Paul J.
author_facet Lager, Angela M.
Corradin, Olivia G.
Cregg, Jared M.
Elitt, Matthew S.
Shick, H. Elizabeth
Clayton, Benjamin L. L.
Allan, Kevin C.
Olsen, Hannah E.
Madhavan, Mayur
Tesar, Paul J.
author_sort Lager, Angela M.
collection PubMed
description Oligodendrocyte dysfunction underlies many neurological disorders, but rapid assessment of mutation-specific effects in these cells has been impractical. To enable functional genetics in oligodendrocytes, here we report a highly efficient method for generating oligodendrocytes and their progenitors from mouse embryonic and induced pluripotent stem cells, independent of mouse strain or mutational status. We demonstrate that this approach, when combined with genome engineering, provides a powerful platform for the expeditious study of genotype–phenotype relationships in oligodendrocytes.
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spelling pubmed-61372092018-09-17 Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells Lager, Angela M. Corradin, Olivia G. Cregg, Jared M. Elitt, Matthew S. Shick, H. Elizabeth Clayton, Benjamin L. L. Allan, Kevin C. Olsen, Hannah E. Madhavan, Mayur Tesar, Paul J. Nat Commun Article Oligodendrocyte dysfunction underlies many neurological disorders, but rapid assessment of mutation-specific effects in these cells has been impractical. To enable functional genetics in oligodendrocytes, here we report a highly efficient method for generating oligodendrocytes and their progenitors from mouse embryonic and induced pluripotent stem cells, independent of mouse strain or mutational status. We demonstrate that this approach, when combined with genome engineering, provides a powerful platform for the expeditious study of genotype–phenotype relationships in oligodendrocytes. Nature Publishing Group UK 2018-09-13 /pmc/articles/PMC6137209/ /pubmed/30213958 http://dx.doi.org/10.1038/s41467-018-06102-7 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Lager, Angela M.
Corradin, Olivia G.
Cregg, Jared M.
Elitt, Matthew S.
Shick, H. Elizabeth
Clayton, Benjamin L. L.
Allan, Kevin C.
Olsen, Hannah E.
Madhavan, Mayur
Tesar, Paul J.
Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells
title Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells
title_full Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells
title_fullStr Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells
title_full_unstemmed Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells
title_short Rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells
title_sort rapid functional genetics of the oligodendrocyte lineage using pluripotent stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6137209/
https://www.ncbi.nlm.nih.gov/pubmed/30213958
http://dx.doi.org/10.1038/s41467-018-06102-7
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