Chemical Modification of CRISPR gRNAs Eliminate type I Interferon Responses in Human Peripheral Blood Mononuclear Cells

OBJECTIVES: CRISPR/Cas9 is currently the primary tool used for genome editing in mammalian cells. To cleave and alter genomic DNA, both the Cas9 nuclease and a guide RNA (gRNA) must be present in the nucleus. One preferred method of introducing these reagents is direct transfection of a recombinant...

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Detalles Bibliográficos
Autores principales: Schubert, Mollie S., Cedrone, Edward, Neun, Barry, Behlke, Mark A., Dobrovolskaia, Marina A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6138052/
https://www.ncbi.nlm.nih.gov/pubmed/30225466
http://dx.doi.org/10.4172/2576-3881.1000121

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6138052/
https://www.ncbi.nlm.nih.gov/pubmed/30225466
http://dx.doi.org/10.4172/2576-3881.1000121

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