Enhanced uptake of gH625 by blood brain barrier compared to liver in vivo: characterization of the mechanism by an in vitro model and implications for delivery

We have investigated the crossing of the blood brain barrier (BBB) by the peptide gH625 and compared to the uptake by liver in vivo. We clearly observed that in vivo administration of gH625 allows the crossing of the BBB, although part of the peptide is sequestered by the liver. Furthermore, we used...

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Autores principales: Falanga, Annarita, Iachetta, Giuseppina, Lombardi, Lucia, Perillo, Emiliana, Lombardi, Assunta, Morelli, Giancarlo, Valiante, Salvatore, Galdiero, Stefania
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6138628/
https://www.ncbi.nlm.nih.gov/pubmed/30218088
http://dx.doi.org/10.1038/s41598-018-32095-w
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author Falanga, Annarita
Iachetta, Giuseppina
Lombardi, Lucia
Perillo, Emiliana
Lombardi, Assunta
Morelli, Giancarlo
Valiante, Salvatore
Galdiero, Stefania
author_facet Falanga, Annarita
Iachetta, Giuseppina
Lombardi, Lucia
Perillo, Emiliana
Lombardi, Assunta
Morelli, Giancarlo
Valiante, Salvatore
Galdiero, Stefania
author_sort Falanga, Annarita
collection PubMed
description We have investigated the crossing of the blood brain barrier (BBB) by the peptide gH625 and compared to the uptake by liver in vivo. We clearly observed that in vivo administration of gH625 allows the crossing of the BBB, although part of the peptide is sequestered by the liver. Furthermore, we used a combination of biophysical techniques to gain insight into the mechanism of interaction with model membranes mimicking the BBB and the liver. We observed a stronger interaction for membranes mimicking the BBB where gH625 clearly undergoes a change in secondary structure, indicating the key role of the structural change in the uptake mechanism. We report model studies on liposomes which can be exploited for the optimization of delivery tools.
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spelling pubmed-61386282018-09-15 Enhanced uptake of gH625 by blood brain barrier compared to liver in vivo: characterization of the mechanism by an in vitro model and implications for delivery Falanga, Annarita Iachetta, Giuseppina Lombardi, Lucia Perillo, Emiliana Lombardi, Assunta Morelli, Giancarlo Valiante, Salvatore Galdiero, Stefania Sci Rep Article We have investigated the crossing of the blood brain barrier (BBB) by the peptide gH625 and compared to the uptake by liver in vivo. We clearly observed that in vivo administration of gH625 allows the crossing of the BBB, although part of the peptide is sequestered by the liver. Furthermore, we used a combination of biophysical techniques to gain insight into the mechanism of interaction with model membranes mimicking the BBB and the liver. We observed a stronger interaction for membranes mimicking the BBB where gH625 clearly undergoes a change in secondary structure, indicating the key role of the structural change in the uptake mechanism. We report model studies on liposomes which can be exploited for the optimization of delivery tools. Nature Publishing Group UK 2018-09-14 /pmc/articles/PMC6138628/ /pubmed/30218088 http://dx.doi.org/10.1038/s41598-018-32095-w Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Falanga, Annarita
Iachetta, Giuseppina
Lombardi, Lucia
Perillo, Emiliana
Lombardi, Assunta
Morelli, Giancarlo
Valiante, Salvatore
Galdiero, Stefania
Enhanced uptake of gH625 by blood brain barrier compared to liver in vivo: characterization of the mechanism by an in vitro model and implications for delivery
title Enhanced uptake of gH625 by blood brain barrier compared to liver in vivo: characterization of the mechanism by an in vitro model and implications for delivery
title_full Enhanced uptake of gH625 by blood brain barrier compared to liver in vivo: characterization of the mechanism by an in vitro model and implications for delivery
title_fullStr Enhanced uptake of gH625 by blood brain barrier compared to liver in vivo: characterization of the mechanism by an in vitro model and implications for delivery
title_full_unstemmed Enhanced uptake of gH625 by blood brain barrier compared to liver in vivo: characterization of the mechanism by an in vitro model and implications for delivery
title_short Enhanced uptake of gH625 by blood brain barrier compared to liver in vivo: characterization of the mechanism by an in vitro model and implications for delivery
title_sort enhanced uptake of gh625 by blood brain barrier compared to liver in vivo: characterization of the mechanism by an in vitro model and implications for delivery
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6138628/
https://www.ncbi.nlm.nih.gov/pubmed/30218088
http://dx.doi.org/10.1038/s41598-018-32095-w
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