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rec-YnH enables simultaneous many-by-many detection of direct protein–protein and protein–RNA interactions

Knowing which proteins and RNAs directly interact is essential for understanding cellular mechanisms. Unfortunately, discovering such interactions is costly and often unreliable. To overcome these limitations, we developed rec-YnH, a new yeast two and three-hybrid-based screening pipeline capable of...

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Detalles Bibliográficos
Autores principales: Yang, Jae-Seong, Garriga-Canut, Mireia, Link, Nele, Carolis, Carlo, Broadbent, Katrina, Beltran-Sastre, Violeta, Serrano, Luis, Maurer, Sebastian P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6138660/
https://www.ncbi.nlm.nih.gov/pubmed/30217970
http://dx.doi.org/10.1038/s41467-018-06128-x